Product Description
REAGEN Aflatoxin B1 ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Aflatoxin B1 in cereals, feed, milk, milk powder,enzyme,cookies, soy sauce, vinegar, oils, peanuts .
The unique features of the kit are:
- High recovery (75-125%),rapid(10-40minutes), cost-effective extraction methods.
- High sensitivity (0.02ng/g or ppb).
- High reproducibility.
- A quick ELISA assay (only 1 hourregardless of number of samples).
Procedure Overview
REAGEN The method is based on a competitive colorimetric ELISA assay. The Aflatoxin B1-BSA has been coated in the plate wells. During the analysis, sample and Aflatoxin B1 antibody (Antibody#1) and HRP-Conjugate (HRP-Conjugated Antibody #2) are added to the wells for incubation. If the Aflatoxin B1 residue is present in the sample, it will compete with the Aflatoxin B1 on the plate wells for the Aflatoxin B1 antibody, The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the Aflatoxin B1 residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN Aflatoxin B1 ELISA Test Kit has the Aflatoxin B1acity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8℃ *. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Aflatoxin B1-BSA-Coated Plate | 1x 96-well Plate (8 wells x 12 strips) |
2-8℃
2-8℃ |
| Aflatoxin B1 Standards:
Negative control (white cap tube) 0.02ng/mL (yellow cap tube) 0.06 ng/mL (orange cap tube) 0.2 ng/mL (pink cap tube) 0.6 ng/mL (purple cap tube) 1.5 ng/mL (blue cap tube) |
1.0 mL 1.0.mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL |
|
| Antibody #1 | 6 mL | |
| HRP-Conjugated Antibody #2 | 6 mL | |
| 20X Wash Solution | 30 mL | |
| 10 X Sample Dilution Buffer | 25mL | |
| Stop Buffer | 12 mL | |
| TMB Substrate | 12 mL |
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ng/g or ppb) |
| Milk/Milk Powder，Enzyme | 0.04/0.1 |
| Cereal/Feed/Seed/Cookies | 1.0 |
| Vegatable/bean/peanut/oils | 0.2 |
| Chili powder/Soy sauce/vinegar | 0.5 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Aflatoxin B1 | 100.0 |
| Aflatoxin B2 | 49 |
| Aflatoxin G1 | 37 |
| Aflatoxin G2 | 8 |
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