Product Description
REAGEN Vitamin B5 Test Kit is quantitative analysis of total Vitamin B5 in food ,feed and drug.
The unique features of the kit are:
- rapid, and cost-effective extraction methods,the same as other Vitamin.
- The kit provide positive control and serial standards
- High sensitivity (80μg/100g)
- High recovery(80%-120%)
- High reproducibility(Intra-assay CV for standards: < 12%,Inter-assay CV for standards: < 15 %
Procedure Overview
REAGEN Sample and Vitamin B5 culture medium add to the 96-microwell which has the Lactobacillus plantarum, if the sample has Vitamin B5 the Lactobacillus plantarum will grow, after culture 37℃,44-48h, read the OD by microplate reader 610 – 630 nm (or540 – 550 nm),The sample of the turbidity compared with standard curve can get Vitamin B5 content.
Kit Contents, Storage and Shelf Life
REAGEN Vitamin B5 Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8℃ .The shelf life is 6 months when the kit is properly stored.
Kit Contents | Amount | Storage |
Lactobacillus plantarum -Coated Plate | 1 x 96-well Plate (8 wells x 12 strips) | 2-8℃ |
Sterile water | 3x30ml | 2-8℃ |
Vitamin B5 Culture medium | 3 | 2-8℃ |
Vitamin B5 standards | 3 | 2-8℃ |
Aluminum foil | 2 | 2-30℃ |
Required Materials Not Provided With the Kit
- Laminar flow bench
- Microtiter plate reader:610 – 630 nm (540 – 550 nm)
- Incubator:37 °C
- Water bath 95℃
- Centrifuge:8000g
- Sterile Pipette 20-200ul,100-1000ul
- Sterile centrifuge tubes (1.5-2ml ,15 and 50 mL)
- The 500 mL glass bottles and flasks (100 and 1000 mL), 100 mL beaker
- Sterile syringe, 0.2 um sterile membrane
- Sterile water ordeionized water
- Amylasez:B.Fluka 86250
- H2SO4:1mol/L
- NaOH concentrate(take 40g NaOH add 100ml Sterile water or deionized water)
and 2mol/L NaOH(take 8g NaOH add 100ml Sterile water or deionized water)
- NaOH:1mol/L and 0.1mol/L
- HCl:1mol/L and 0.1mol/L
- Citrate buffer(pH5),take 1.5 g citric acid monohydrate to 100 ml beaker , add 50 ml sterile water or deionized water, stirring and dissolved, then add 12 ml NaOH (1 mol/L) or 0.48 g NaOH, using 0.1 mol/L HCl to pH to 4.5;With sterile water or deionized water transferring quantitative solution into 100 ml volumetric flask, with sterile water or deionized water constant volume, can be stored three days in 2-8 ℃.
Reviews
There are no reviews yet.