Product Description
REAGEN Total Aflatoxin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Total Aflatoxin in cereals, feed, milk, milk powder,enzyme,cookies, soy sauce, vinegar, oils, peanuts,liver,egg .
The unique features of the kit are:
- High recovery (75-125%),rapid(10-40minutes), cost-effective extraction methods.
- Detection Limitis 1 ng/g .
- High reproducibility.
- A quick ELISA assay (only 1 hourregardless of number of samples).
Procedure Overview
REAGEN The method is based on a competitive colorimetric ELISA assay. The Total Aflatoxin-BSA has been coated in the plate wells. During the analysis, sample and Total Aflatoxin antibody (Antibody#1) and HRP-Conjugate (HRP-Conjugated Antibody #2) are added to the wells for incubation. If the Total Aflatoxin residue is present in the sample, it will compete with the Total Aflatoxin on the plate wells for the Total Aflatoxin antibody, The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the Total Aflatoxin residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN Total Aflatoxin ELISA Test Kit has the Total Aflatoxinacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8℃ *. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Total Aflatoxin-BSA-Coated Plate | 1x 96-well Plate (8 wells x 12 strips) |
2-8℃
2-8℃ |
| Total Aflatoxin Standards:
Negative control (white cap tube) 0.02ng/mL (yellow cap tube) 0.06 ng/mL (orange cap tube) 0.2 ng/mL (pink cap tube) 0.6 ng/mL (purple cap tube) 1.5 ng/mL (blue cap tube) |
1.0 mL 1.0.mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL |
|
| Antibody #1 | 6 mL | |
| HRP-Conjugated Antibody #2 | 6 mL | |
| 20X Wash Solution | 30 mL | |
| 10 X Sample Dilution Buffer | 25mL | |
| Stop Buffer | 12 mL | |
| TMB Substrate | 12 mL |
Detection Limit Of Quantitation
| Sample Type | Limit Of Quantitation (ng/g ) |
| Milk/Milk Powder，Enzyme | 0.04/0.1 |
| Cereal/Feed/Seed/Cookies/Soy sauce/vinegar | 0.5 |
| Vegatable/bean/peanut/oils | 0.2 |
| Egg/ liver | 0.5 |
Specificity
| Analytes | Cross-Reactivity (%) |
| Aflatoxin B1 | 100.0 |
| Aflatoxin B2 | 76 |
| Aflatoxin M1 | 79 |
| Aflatoxin M2 | 33 |
| Aflatoxin G1 | 55 |
| Aflatoxin G2 | 6 |
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