Total Aflatoxin ELISA Test Kit

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Total Aflatoxin ELISA Test Kit

$999.00

Total Aflatoxin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Total Aflatoxin in cereals, feed, milk, milk powder,enzyme,cookies, soy sauce, vinegar, oils, peanuts,liver,egg .

Availability: 100 in stock (can be backordered)

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Product Description

REAGEN  Total Aflatoxin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Total Aflatoxin in cereals, feed, milk, milk powder,enzyme,cookies, soy sauce, vinegar, oils, peanuts,liver,egg .

The unique features of the kit are:

  • High recovery (75-125%),rapid(10-40minutes), cost-effective extraction methods.
  • Detection Limitis 1 ng/g .
  • High reproducibility.
  • A quick ELISA assay (only 1 hourregardless of number of samples).

Procedure Overview

REAGEN  The method is based on a competitive colorimetric ELISA assay. The Total Aflatoxin-BSA has been coated in the plate wells. During the analysis, sample and Total Aflatoxin antibody (Antibody#1) and HRP-Conjugate (HRP-Conjugated Antibody #2) are added to the wells for incubation. If the Total Aflatoxin residue is present in the sample, it will compete with the Total Aflatoxin on the plate wells for the Total Aflatoxin antibody, The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the Total Aflatoxin residue concentration in the sample.

Kit Contents, Storage and Shelf Life

 REAGEN   Total Aflatoxin ELISA Test Kit has the Total Aflatoxinacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8℃ *. The shelf life is 12 months when the kit is properly stored.

Kit Contents Amount Storage
Total Aflatoxin-BSA-Coated Plate 1x 96-well Plate (8 wells x 12 strips)  

2-8℃

 

 

 

 

 

2-8℃

Total Aflatoxin Standards:

Negative control (white cap tube)

0.02ng/mL (yellow cap tube)

0.06 ng/mL (orange cap tube)

0.2 ng/mL (pink cap tube)

0.6 ng/mL (purple cap tube)

1.5 ng/mL (blue cap tube)

 

1.0 mL

1.0.mL

1.0 mL

1.0 mL

1.0 mL

1.0 mL

Antibody #1 6 mL
HRP-Conjugated Antibody #2 6 mL
20X Wash Solution 30 mL
10 X Sample Dilution Buffer 25mL
Stop Buffer 12 mL
TMB Substrate 12 mL

Detection Limit Of Quantitation

Sample Type Limit Of Quantitation (ng/g )
Milk/Milk Powder,Enzyme 0.04/0.1
Cereal/Feed/Seed/Cookies/Soy sauce/vinegar 0.5
Vegatable/bean/peanut/oils 0.2
Egg/ liver 0.5

Specificity

Analytes Cross-Reactivity (%)
Aflatoxin B1 100.0
Aflatoxin B2 76
Aflatoxin M1 79
Aflatoxin M2 33
Aflatoxin G1 55
Aflatoxin G2 6
Brand

REAGEN

Model

RNM98006

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IMG_20230915_174149Total Aflatoxin ELISA Test Kit
$999.00

Availability: 100 in stock (can be backordered)